Master Syllabus MT2306, MLT Pathogenic Microbiology

Credits 6 Lab Hours 3 Lecture Hours 3 Clinical Hours 0

Normal flora and pathogenic bacteria will be identified by morphology, staining characteristics, colonial morphology, growth on selective media, biochemical testing and serological methods. Basic theory in antimicrobial susceptibility testing will be covered. Principles of all tests will be studied. Study of viruses will be limited to the processing and handling of specimens for consultant referral and principles of serological testing. Normal and pathogenic parasites and fungal elements will be identified, and procedures utilized for proper identification will be discussed.

For each unit of credit, a minimum of three hours per week with one of the hours for class and two hours for studying/preparation outside of class is expected.

Program and/or Department Mission Statement

The Seward County Community College Medical Laboratory Technology program provides a curriculum that produces competent, career entry level medical laboratory technicians.

SCCC Outcomes

Outcome #1: Read with comprehension, be critical of what they read, and apply knowledge gained from their reading to broader issues of the day.

Outcome #2: Communicate ideas clearly and proficiently in writing, appropriately adjusting content and arrangement for varying audiences, purposes, and situations.

Outcome #3: Communicate their ideas clearly and proficiently in speaking, appropriately adjusting content and arrangement for varying audiences, purposes, and situations

Outcome #4: Demonstrate mathematical skills by using a variety of techniques and technologies.

Outcome #5: Demonstrate the ability to think critically by gathering facts, generating insights, analyzing data, and evaluating information.

Outcome #6: Exhibit skills in information and technological literacy.

Outcome #9: Exhibit workplace skills to include respect for others, teamwork competence, attendance/punctuality, decision making, conflict resolution, truthfulness/honest, positive attitude, judgment, responsibility.

Course Outcomes

Upon completion of this course, the student will be able to:

analytical errors for each of the analytes discussed or approached in the course.

confidentiality.

human pathogenic bacteria with the collection and processing of clinical

specimens for bacteriological culture.

including microscopic, staining, plating, sub-culturing, and identification

techniques.

selective media to determine initial grouping of bacteria commonly encountered in clinical specimens to determine identification steps.

pathogenic organisms. Briefly discuss the characteristics that would be used to identify the organism.

  1. Relate the proper specimen collection and handling, type of qualify control used, reference ranges, principle of analysis currently available, and sources of
  2. Perform all procedures with regard to prescribed safety protocol and
  3. Correlate abnormal results with the most likely disease process by determining the clinical significance of the findings.
  4. Relate the transmission, entry into their host, and disease mechanism of common
  5. Describe the appropriate methods of collecting clinical specimens given the body site and the type of organism that is suspected.
  6. Describe and perform accurately and safely basic microbiology techniques
  7. Relate the physical and biochemical growth requirements of specific bacteria to the composition of general, selective and identification media.
  8. Utilize simple and compound stains, colony characteristics, and growth on
  9. Identify common normal flora and possible pathogens from clinical specimens by the utilization of biochemical testing. Relate the principle behind each test utilized.
  10. Relate the method of transport of either culture or specimen for unusual
Course Outline

** FOR EACH GROUP OF ORGANISMS STUDIED THE FOLLOWING WILL APPLY:

a. methods

b. principles

c. interpretation

a. disease states

b. normal flora

c. treatment

  1. Laboratory Safety
  2. Quality in the Clinical Microbiology Laboratory
  3. Specimen Management
  4. Bloodstream Infections
  5. Host-Microorganism Interactions
  6. Traditional Cultivation and Identification.
  7. Nucleic Acid Based Analytic Methods
  8. Staphylococcus, Micrococcus
  9. Streptococcus, Enterococcus
  10. Neisseriae, Moraxella
  11. Haemophilus
  12. Enterobacteriaceae
  13. Pseudomonas, Burkholderia
  14. Acinetobacter, Aggregatibacter, Kingella, Cardiobacterium, Capnocytophaga
  15. Lab Methods and Strategies for AST
  16. Vibrio, Aeromonas, Chromobacterium
  17. Campylobacter, Helicobacter
  18. Legionella
  19. Bordetalla pertussis and parapertussis
  20. Eikenella
  21. Pasturella
  22. Francisella
  23. Erysipelothrix, Lactobacillus, Gardnerella
  24. Bacillus
  25. Listeria, Corynebacterium
  26. Spirochetes
  27. Chlamydia
  28. Anaerobes
  29. Mycobacteria
  30. Virology
  31. Parasitology
  32. Mycology
  33. Classification of organisms within the group
  34. Special considerations for primary isolation
  35. Colonial and cellular morphology
  36. Identification protocol
  37. Clinical significance of each organism
  38. Sensitivity patterns
Instructional Methods

Lecture, laboratory exercises, demonstration, simulation, and computer tutorials.

Instructional Resources and Materials

Handouts, laboratory equipment and supplies, selected reference readings.

Methods of Assessment

SCCC Outcome 1 will be assessed and measured by class participation and writing assignments indicating comprehension of the material read.

SCCC Outcome 2 will be assessed and measured by written laboratory reports.

SCCC Outcome 3 will be assessed and measured by verbal communication with clinical instructors and of laboratory reports.

SCCC Outcome 4 will be assessed by the students'� ability to correctly perform clinical laboratory calculations.

SCCC Outcome 5 will be assessed and measured by the students'� ability to correctly process cultures, determine validity of results and correlate with possible pathogenic organisms.

Students will also be assessed on their ability to follow prescribed procedures for troubleshooting and problem solving.

SCCC Outcome 6 will be assessed and measured by the students'� ability to properly and efficiently operate automated equipment and the microscope in the clinical site labs and by the student'�s ability to locate and review articles(s) from professional publications relevant to the specified course work.

SCCC Outcome 9 will be assessed and measured by the completion of the Student Attitude Assessment tools by didactic and clinical instructors.

Americans with Disabilities Act (ADA) Statement

Under the Americans with Disabilities Act, Seward County Community College will make reasonable accommodations for students with documented disabilities. If you need support or assistance because of a disability, you may be eligible for academic accommodations. Students should identify themselves to the Mental Health Counselor at 620-417-1106 or go to the Student Success Center in the Hobble Academic building, room A149.